ENCODE DNAse Summits Track Settings
 
ENCODE DNAse Hypersensitivity (125 cell types) v3   (All Histone Marks & DNAse Hypersensitivity tracks)

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 8988t  ENCODE_DNAse_Coverage 8988t   Schema 
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 A549  ENCODE_DNAse_Coverage A549   Schema 
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 Adult CD4  ENCODE_DNAse_Coverage Adult CD4   Schema 
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 AG04449  ENCODE_DNAse_Coverage AG04449   Schema 
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 AG04450  ENCODE_DNAse_Coverage AG04450   Schema 
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 AG09309  ENCODE_DNAse_Coverage AG09309   Schema 
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 AG09319  ENCODE_DNAse_Coverage AG09319   Schema 
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 AG10803  ENCODE_DNAse_Coverage AG10803   Schema 
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 AoAF  ENCODE_DNAse_Coverage AoAF   Schema 
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 AoSMC  ENCODE_DNAse_Coverage AoSMC   Schema 
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 BE2 C  ENCODE_DNAse_Coverage BE2 C   Schema 
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 BJ  ENCODE_DNAse_Coverage BJ   Schema 
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 Caco-2  ENCODE_DNAse_Coverage Caco-2   Schema 
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 CD14+  ENCODE_DNAse_Coverage CD14+   Schema 
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 CD20+  ENCODE_DNAse_Coverage CD20+   Schema 
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 CD34+ Mobilized  ENCODE_DNAse_Coverage CD34+ Mobilized   Schema 
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 Chorion  ENCODE_DNAse_Coverage Chorion   Schema 
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 Cll  ENCODE_DNAse_Coverage Cll   Schema 
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 CMK  ENCODE_DNAse_Coverage CMK   Schema 
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 Fibrobl  ENCODE_DNAse_Coverage Fibrobl   Schema 
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 Fibrop  ENCODE_DNAse_Coverage Fibrop   Schema 
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 Gliobla  ENCODE_DNAse_Coverage Gliobla   Schema 
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 GM06990  ENCODE_DNAse_Coverage GM06990   Schema 
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 GM12864  ENCODE_DNAse_Coverage GM12864   Schema 
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 GM12865  ENCODE_DNAse_Coverage GM12865   Schema 
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 GM12878  ENCODE_DNAse_Coverage GM12878   Schema 
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 Gm12891  ENCODE_DNAse_Coverage Gm12891   Schema 
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 Gm12892  ENCODE_DNAse_Coverage Gm12892   Schema 
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 Gm18507  ENCODE_DNAse_Coverage Gm18507   Schema 
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 Gm19238  ENCODE_DNAse_Coverage Gm19238   Schema 
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 Gm19239  ENCODE_DNAse_Coverage Gm19239   Schema 
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 Gm19240  ENCODE_DNAse_Coverage Gm19240   Schema 
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 H1-hESC  ENCODE_DNAse_Coverage H1-hESC   Schema 
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 H9es  ENCODE_DNAse_Coverage H9es   Schema 
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 HA-h  ENCODE_DNAse_Coverage HA-h   Schema 
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 HA-sp  ENCODE_DNAse_Coverage HA-sp   Schema 
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 HAc  ENCODE_DNAse_Coverage HAc   Schema 
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 HAEpiC  ENCODE_DNAse_Coverage HAEpiC   Schema 
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 HBMEC  ENCODE_DNAse_Coverage HBMEC   Schema 
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 HCF  ENCODE_DNAse_Coverage HCF   Schema 
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 HCFaa  ENCODE_DNAse_Coverage HCFaa   Schema 
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 HCM  ENCODE_DNAse_Coverage HCM   Schema 
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 HConF  ENCODE_DNAse_Coverage HConF   Schema 
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 HCPEpiC  ENCODE_DNAse_Coverage HCPEpiC   Schema 
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 HCT-116  ENCODE_DNAse_Coverage HCT-116   Schema 
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 HEEpiC  ENCODE_DNAse_Coverage HEEpiC   Schema 
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 HeLa-S3  ENCODE_DNAse_Coverage HeLa-S3   Schema 
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 HeLa-S3s3Ifna4h  ENCODE_DNAse_Coverage HeLa-S3s3Ifna4h   Schema 
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 Hepatocytes  ENCODE_DNAse_Coverage Hepatocytes   Schema 
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 HepG2  ENCODE_DNAse_Coverage HepG2   Schema 
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 HFF  ENCODE_DNAse_Coverage HFF   Schema 
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 HFF-Myc  ENCODE_DNAse_Coverage HFF-Myc   Schema 
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 HGF  ENCODE_DNAse_Coverage HGF   Schema 
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 HIPEpiC  ENCODE_DNAse_Coverage HIPEpiC   Schema 
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 HL-60  ENCODE_DNAse_Coverage HL-60   Schema 
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 HMEC  ENCODE_DNAse_Coverage HMEC   Schema 
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 HMF  ENCODE_DNAse_Coverage HMF   Schema 
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 HMVEC-dAd  ENCODE_DNAse_Coverage HMVEC-dAd   Schema 
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 HMVEC-dBl-Ad  ENCODE_DNAse_Coverage HMVEC-dBl-Ad   Schema 
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 HMVEC-dBl-Neo  ENCODE_DNAse_Coverage HMVEC-dBl-Neo   Schema 
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 HMVEC-dLy-Ad  ENCODE_DNAse_Coverage HMVEC-dLy-Ad   Schema 
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 HMVEC-dLy-Neo  ENCODE_DNAse_Coverage HMVEC-dLy-Neo   Schema 
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 HMVEC-dNeo  ENCODE_DNAse_Coverage HMVEC-dNeo   Schema 
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 HMVEC-LBl  ENCODE_DNAse_Coverage HMVEC-LBl   Schema 
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 HMVEC-LLy  ENCODE_DNAse_Coverage HMVEC-LLy   Schema 
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 HNPCEpiC  ENCODE_DNAse_Coverage HNPCEpiC   Schema 
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 HPAEC  ENCODE_DNAse_Coverage HPAEC   Schema 
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 HPAF  ENCODE_DNAse_Coverage HPAF   Schema 
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 HPDE6-E6E7  ENCODE_DNAse_Coverage HPDE6-E6E7   Schema 
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 HPdLF  ENCODE_DNAse_Coverage HPdLF   Schema 
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 HPF  ENCODE_DNAse_Coverage HPF   Schema 
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 HRCEpiC  ENCODE_DNAse_Coverage HRCEpiC   Schema 
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 HRE  ENCODE_DNAse_Coverage HRE   Schema 
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 HRGEC  ENCODE_DNAse_Coverage HRGEC   Schema 
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 HRPEpiC  ENCODE_DNAse_Coverage HRPEpiC   Schema 
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 HSMM  ENCODE_DNAse_Coverage HSMM   Schema 
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 HSMM emb  ENCODE_DNAse_Coverage HSMM emb   Schema 
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 HSMM FSHD  ENCODE_DNAse_Coverage HSMM FSHD   Schema 
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 HTR8svn  ENCODE_DNAse_Coverage HTR8svn   Schema 
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 Huh-7  ENCODE_DNAse_Coverage Huh-7   Schema 
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 HUVEC  ENCODE_DNAse_Coverage HUVEC   Schema 
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 HVMF  ENCODE_DNAse_Coverage HVMF   Schema 
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 Ips  ENCODE_DNAse_Coverage Ips   Schema 
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 Ishikawa  ENCODE_DNAse_Coverage Ishikawa   Schema 
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 Jurkat  ENCODE_DNAse_Coverage Jurkat   Schema 
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 K562  ENCODE_DNAse_Coverage K562   Schema 
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 LNCaP  ENCODE_DNAse_Coverage LNCaP   Schema 
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 LNCap  ENCODE_DNAse_Coverage LNCap   Schema 
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 MCF-7  ENCODE_DNAse_Coverage MCF-7   Schema 
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 Medullo  ENCODE_DNAse_Coverage Medullo   Schema 
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 Melano  ENCODE_DNAse_Coverage Melano   Schema 
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 Myometr  ENCODE_DNAse_Coverage Myometr   Schema 
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 NB4  ENCODE_DNAse_Coverage NB4   Schema 
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 NH-A  ENCODE_DNAse_Coverage NH-A   Schema 
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 NHDF-Ad  ENCODE_DNAse_Coverage NHDF-Ad   Schema 
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 NHDF-neo  ENCODE_DNAse_Coverage NHDF-neo   Schema 
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 NHEK  ENCODE_DNAse_Coverage NHEK   Schema 
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 NHLF  ENCODE_DNAse_Coverage NHLF   Schema 
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 NT2-D1  ENCODE_DNAse_Coverage NT2-D1   Schema 
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 Osteobl  ENCODE_DNAse_Coverage Osteobl   Schema 
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 PANC-1  ENCODE_DNAse_Coverage PANC-1   Schema 
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 PanIsletD  ENCODE_DNAse_Coverage PanIsletD   Schema 
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 Panislets  ENCODE_DNAse_Coverage Panislets   Schema 
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 Phte  ENCODE_DNAse_Coverage Phte   Schema 
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 PrEC  ENCODE_DNAse_Coverage PrEC   Schema 
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 Progfib  ENCODE_DNAse_Coverage Progfib   Schema 
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 RPTEC  ENCODE_DNAse_Coverage RPTEC   Schema 
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 RWPE1  ENCODE_DNAse_Coverage RWPE1   Schema 
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 SAEC  ENCODE_DNAse_Coverage SAEC   Schema 
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 SK-N-MC  ENCODE_DNAse_Coverage SK-N-MC   Schema 
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 SK-N-SH  ENCODE_DNAse_Coverage SK-N-SH   Schema 
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 SkMC  ENCODE_DNAse_Coverage SkMC   Schema 
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 Stellate  ENCODE_DNAse_Coverage Stellate   Schema 
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 T-47D  ENCODE_DNAse_Coverage T-47D   Schema 
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 Th1  ENCODE_DNAse_Coverage Th1   Schema 
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 Th2  ENCODE_DNAse_Coverage Th2   Schema 
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 Urothelia  ENCODE_DNAse_Coverage Urothelia   Schema 
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 UrotheliaUt189  ENCODE_DNAse_Coverage UrotheliaUt189   Schema 
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 WERI-Rb-1  ENCODE_DNAse_Coverage WERI-Rb-1   Schema 
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 WI-38  ENCODE_DNAse_Coverage WI-38   Schema 
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 WI-38 TAM  ENCODE_DNAse_Coverage WI-38 TAM   Schema 
    

Description

This track shows the track for DNAse I Hypersensitivity sites for cell types from the hg38 DNaseI Hypersensitive Site Master List (125 cell types) from ENCODE/Analysis lifted to the Repeat Browser

Display Conventions and Configuration

Individual instances from the ENCODE DnasI hypersensitivity track are shown aligned to the Repeat Browser consensus. Peaks indicate that this region is hypersensitive at most instances of this repeat type across the genome.

Use in combination with the corresponding meta-summit and instance mapping tracks. Chromosome coordinates (hg38) shown for each peak.

Methods

wget http://hgdownload.soe.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeAwgDnaseMasterSites/wgEncodeAwgDnaseMasterSites.bed.gz

gunzip *.gz

wget http://hgdownload.soe.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeAwgDnaseMasterSites/wgEncodeAwgDnaseMasterSources.tab

awk -v OFS="\t" '{print $1,$2,$3,$1":"$2"-"$3"*"$8,$5,"+"}' wgEncodeAwgDnaseMasterSites.bed > encode_DNAse_5.bed

liftOver -multiple encode_DNAse_5.bed ../lift/hg19_to_hg38reps.over.chain encode_DNAse_5_hg38reps.bed encode_DNAse_5_hg38reps.unmapped

awk -v OFS="\t" '{split($4, a, "*"); {print $1,$2,$3,a[1],$5,"+",a[2]}}' encode_DNAse_5_hg38reps.bed > temp.bed

awk -v OFS="\t" '{split($7, a, ","); for(i in a) { if (a[i] ~/^[0-9]+$/ ){print $1,$2,$3,$4,$5,"+",a[i]}}}' temp.bed | sort -k 7b,7 > encode_DNAse_7_hg38reps.bed

sort -k1b,1 wgEncodeAwgDnaseMasterSources.tab > DNAse_key.tab

join -1 1 -2 7 DNAse_key.tab encode_DNAse_7_hg38reps.bed | awk -v OFS="\t" '{print $3,$4,$5,$6,$7,$8, $2}' > encode_DNAse_7_hg38reps_named.bed

mv encode_DNAse_7_hg38reps_named.bed ~/hive/jferna10/RepeatBrowserHub/hg38reps/wgEncodeAwgDnaseMasterSites

awk -v OFS="\t" '{print $1,$2,$3,$4,$5,$6 >> "encode_DNAse_6_" $7 ".bed"}' encode_DNAse_7_hg38reps_named.bed

mv encode_DNAse_7_hg38reps_named.bed encode_DNAse_7_hg38reps_named.bak

ls *.bed | cut -f 1 -d"."| while read i; do bedSort $i.bed $i.bed ; bedtools genomecov -bg -split -i $i.bed -g ../hg38reps.sizes > temp.bg; bedGraphToBigWig temp.bg ../hg38reps.sizes $i.bw; done

ls *.bed | cut -f 1 -d"."| while read i; do bedSort $i.bed $i.bed ; bedToBigBed $i.bed ../hg38reps.sizes $i.bb -type=bed6; done

References

DNaseI Hypersensitive Site Master List (125 cell types) from ENCODE/Analysis

Thurman RE, Rynes E, Humbert R, Vierstra J, Maurano MT, Haugen E, Sheffield NC, Stergachis AB, Wang H, Vernot B et al. The accessible chromatin landscape of the human genome. Nature. 2012 Sep 6;489(7414):75-82. PMID: 22955617; PMC: PMC3721348

John S, Sabo PJ, Thurman RE, Sung MH, Biddie SC, Johnson TA, Hager GL, Stamatoyannopoulos JA. Chromatin accessibility pre-determines glucocorticoid receptor binding patterns. Nat Genet. 2011 Mar;43(3):264-8. PMID: 21258342

See also the references and credit sections in the related ENCODE Uniform DnaseI HS, ENCODE UW DnaseI HS and ENCODE Duke DnaseI HS tracks.

Email max@soe.ucsc.edu or jferna10@ucsc.edu