Description
This track shows the meta summits from Imbeault et al 2017. This study was a large scale ChIP-SEQ "screen" of ~200 KRAB Zinc Finger proteins HA tagged and expressed in 293 cells.
Meta summits are initially set to be read at a threshold score of 100 (macs2 summit score).
Use in combination with the corresponding coverage and instance mapping tracks.
Methods
Using the Repeat Browser bed files (see "instance mapping tracks") we used macs2 to call peaks with the following settings
macs2 callpeak -f BED -t --keep-dup all --outdir data/macs2/ --nomodel --call-summits --extsize 30 -n
macs2 summits and peaks can be found in data/macs2 if you would like to load custom tracks with less stringently filtered meta summits.
References
KRAB zinc-finger proteins contribute to the evolution of gene regulatory networks.
Imbeault M1, Helleboid PY1, Trono D1.
Nature. 2017 Mar 23;543(7646):550-554. doi: 10.1038/nature21683. Epub 2017 Mar 8.
Email:jferna10@ucsc.edu or max@soe.ucsc.edu
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